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Effect of mixing on enzymatic hydrolysis of steam ...

May 11, 2011  When scaling up lignocellulose-based ethanol production, the desire to increase the final ethanol titer after fermentation can introduce problems. A high concentration of water-insoluble solids (WIS) is needed in the enzymatic hydrolysis step, resulting in increased viscosity, which can cause mass and heat transfer problems because of poor mixing of the material.

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Effect of mixing on enzymatic hydrolysis of cardboard ...

Apr 01, 2012  The objectives of this study were to investigate the effect of mixing conditions on the yield of enzymatic hydrolysis and the subsequent S/L separation. The results show that mixing conditions (impeller-type, baffles and speed) do indeed have a significant effect.

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Effect of mixing on enzymatic hydrolysis of cardboard ...

hydrolysis, mixing conditions have a considerable impact on the performance of the enzymes. Thus mixing conditions in the hydrolysis tank can also influence the downstream operations and, consequently, the overall economy of the bioethanol process. In this experimental study, four types of impeller, at different hydrolysis conditions were

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(PDF) Effect of mixing on enzymatic hydrolysis of ...

ConclusionsThe objectives of this study were to investigate the effect of mixing conditions on the yield of enzymatic hydrolysis and the subsequent S/L separation. The results show that mixing conditions (impeller-type, baffles and speed) do indeed have a significant effect.

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The effects of mixing on the enzymatic hydrolysis of ...

The effect of mixing on the enzymatic hydrolysis was in part determined by the viscosity of the pretreated biomass. For steam pretreated spruce at low solid loading, decreasing the agitation rate had little effect on the the enzymatic hydrolysis. However, if the viscosity was increased by the addition of a thickening agent, the effect of ...

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Mixing effects on the kinetics of enzymatic hydrolysis of ...

Dec 01, 2019  Mixing effects on the kinetics of hydrolysis. The enzymatic hydrolysis is performed in 100 ml conical flasks with cellulose concentrations of 10, 20, 30, 40 and 50 mg/ml at the optimum enzyme to substrate ratio of 1:15 (w/w) for 1 h for measuring the reaction kinetics.

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The effect of high intensity mixing on the enzymatic ...

of intense mixing and enzymatic hydrolysis on fiber length can be quantified. We find that both mechanical mixing and enzymatic hydrolysis tend to reduce the average fiber length. In addition, there is a synergistic effect in that mixing increases the rate of enzymatic hydrolysis, while hydrolysis reduces biomass viscosity and facilitates ...

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The effect of high intensity mixing on the enzymatic ...

Mar 01, 2011  Prior to mixing or enzymatic hydrolysis, dilute-acid-hydrolyzed corn stover contains a variety of anatomical components that vary in size, shape and aspect ratio. Enzymatic hydrolysis without mixing has little effect on the particle morphology over the duration of reaction times used here.

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Mixing effects on the kinetics and the dynamics of two ...

Jul 01, 2018  Mixing effects on the catalytic conversion of cellulose to biofuel products have been reported earlier (Gaikwad and Chakraborty, 2014). However, the effects of mixing – both macro- and micro-mixing – on the kinetics and the dynamics of two-phase enzymatic hydrolysis of hemicelluloses have not yet been studied.

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Using polyvinylpyrrolidone to enhance the enzymatic ...

Mar 01, 2017  The addition of PEG4600 and PVP8000 had great promoting effects on the enzymatic hydrolysis of three different lignocellulosic substrates. More significantly, PVP8000 had the best promoting effects on the enzymatic hydrolysis of all the three substrates, it meant that PVP is a very effective additive for lignocellulosic enzymatic hydrolysis. 3.1.4.

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Effect of mixing on enzymatic hydrolysis of cardboard ...

hydrolysis, mixing conditions have a considerable impact on the performance of the enzymes. Thus mixing conditions in the hydrolysis tank can also influence the downstream operations and, consequently, the overall economy of the bioethanol process. In this experimental study, four types of impeller, at different hydrolysis conditions were

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The effects of mixing on the enzymatic hydrolysis of ...

determining the effect of mixing on the enzymatic hydrolysis was the level of product inhibition. If the glucose and cellobiose concentrations were high, as during high solid hydrolysis of steam pretreated spruce, low agitation rate had a large negative effect onthe enzymatic hydrolysis.However, if the product concentration was kept low, as

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Effect of mixing on enzymatic hydrolysis of steam ...

May 11, 2011  Effect of mixing on enzymatic hydrolysis of steam-pretreated spruce: a quantitative analysis of conversion and power consumption. Benny Palmqvist, 1 Magnus Wiman, 1 and Gunnar Lidén 1. Benny Palmqvist. 1 Department of Chemical Engineering, Lund University, Box 124, Se-221 00 Lund, Sweden. Find articles by Benny Palmqvist.

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Effect of mixing on enzymatic hydrolysis of cardboard ...

Highlights Mixing in enzymatic hydrolysis of cardboard waste was investigated. Proper mixing improves the glucose yield and the solid–liquid separation. High-intensity mixing should be avoided. High glucose yield increased the specific cake resistance in pressure filtration.

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(PDF) Effect of mixing on enzymatic hydrolysis of steam ...

May 11, 2011  The effect of mixing on the enzymatic hydrolysis was in part determined by the viscosity of the pretreated biomass. For steam pretreated spruce at low solid loading (5% WIS), decreasing the ...

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Effect of mixing on enzymatic hydrolysis of cardboard ...

The effect of mixing on the enzymatic hydrolysis was in part determined by the viscosity of the pretreated biomass. For steam pretreated spruce at low solid loading (5% WIS), decreasing the ...

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Mixing effects on the kinetics and the dynamics of two ...

Mixing effects on the kinetics and the dynamics of two-phase enzymatic hydrolysis of hemicellulose for biofuel production. Dutta SK(1), Chakraborty S(2). Author information: (1)Department of Chemical Engineering, Indian Institute of Technology, Kharagpur 721302, India.

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Does sugar inhibition explain mixing effects in enzymatic ...

Enzymatic hydrolysis of lignocellulose is associated with mixing issues, which are likely to affect process performance. The aim of this study was to investigate how viscosity and sugar inhibition influence the mixing‐dependence in hydrolysis of steam‐pretreated spruce.

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Enzymatic hydrolysis of starch in the presence of cereal ...

The in vitro amylolysis of both granular and cooked maize starch and the diffusion of glucose in the presence of 1% and 2% cereal soluble fibre polysaccharides (arabinoxylan and mixed linkage beta-glucan) were studied at various levels of shear mixing in order to identify potential molecular mechanisms underlying observed glycemia-reducing effects of soluble fibres in vivo.

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Enzymatic hydrolysis of bacterial cellulose in the ...

Nov 08, 2021  2.3 Enzymatic hydrolysis of bacterial cellulose. To evaluate the boosting effect of the CP on the degradation of BC, the enzymatic hydrolysis of the cellulosic substrate was performed using cellulase enzyme with and without the addition of the non-catalytic CP.

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EFFECT OF PRETREATMENT ON ENZYMATIC HYDROLYSIS OF ...

Bagasse and chopped poplar wood were used as substrates for studying the effects of pretreat-ments on enzymatic hydrolysis of cellulose. Pretreatments by acid ,alkali and steam-explosion and enzymatichydrolysis at 50℃ for 24 h were adopted in the experiments, Pretreated materials were analysed by de-terming total sugar,sugar fraction and ...

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Effects of mechanical pretreatments on enzymatic ...

The pretreated substrates were then mixed together for the subsequent enzymatic hydrolysis. This study investigates the enzymatic hydrolysis performance of the mixed substrate of WFs and CRs. It is expected that the combination of mechanical pretreatment and mixing different substrates can be potentially adopted in an integrated biorefinery.

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Effect of hemicellulose and lignin removal on enzymatic ...

T1 - Effect of hemicellulose and lignin removal on enzymatic hydrolysis of steam pretreated corn stover. AU - Öhgren, Karin. AU - Bura, Renata. AU - Saddler, Jack. AU - Zacchi, Guido. PY - 2007. Y1 - 2007. N2 - Ethanol can be produced from lignocellulosic biomass using steam pretreatment followed by enzymatic hydrolysis and fermentation.

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The effect of shaking regime on the rate and extent of ...

In an attempt to elucidate the effect of mixing on the rate and extent of enzymatic hydrolysis of cellulosic substrates, alpha -cellulose was hydrolysed using a commercial cellulase preparation at varying levels of substrate concentration (2.5,5 and 7.5% (w/v)) and by using three shaking regimes: continuous at low-speed (25 rpm), continuous at high-speed (150 rpm) and an intermittent regime ...

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(PDF) Effect of mixing on enzymatic hydrolysis of steam ...

May 11, 2011  The effect of mixing on the enzymatic hydrolysis was in part determined by the viscosity of the pretreated biomass. For steam pretreated spruce at low solid loading (5% WIS), decreasing the ...

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The effects of mixing on the enzymatic hydrolysis of ...

May 11, 2017  The effect of mixing on the enzymatic hydrolysis was in part determined by the viscosity of the pretreated biomass. For steam pretreated spruce at low solid loading, decreasing the agitation rate had little effect on the the enzymatic hydrolysis. However, if the viscosity was increased by the addition of a thickening agent, the effect of ...

Get Price

Mixing Effects on the Kinetics of Enzymatic Hydrolysis of ...

Recently, Gaikwad et al. have performed the enzymatic hydrolysis of Avicel PH 101 and analyzed the effects of mixing on the kinetics and dynamics of the enzymatic hydrolysis of cellulose for ...

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The effect of high intensity mixing on the enzymatic ...

A synergistic effect of mixing and enzymatic hydrolysis occurs: the mixing increases the cellulose conversion rate, whilst the increased conversion eases the mixing. The objective was to evaluate ...

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Effects of mechanical pretreatments on enzymatic ...

The pretreated substrates were then mixed together for the subsequent enzymatic hydrolysis. This study investigates the enzymatic hydrolysis performance of the mixed substrate of WFs and CRs. It is expected that the combination of mechanical pretreatment and mixing different substrates can be potentially adopted in an integrated biorefinery.

Get Price

Optimization and scale-up of enzymatic hydrolysis of wood ...

Effect of mixing mode on enzymatic hydrolysis and sugar production. (A and B) pH of manual intermittent mixing and propeller continuous mixing, respectively; (C and D) sugar production of manual intermittent mixing and propeller continuous mixing. The pulp concentration was 15%, and the enzyme dosage was 15 mg/g dry mass of each one of CMax and ...

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(PDF) The effect of shaking regime on the rate and extent ...

In an attempt to elucidate the effect of mixing on the rate and extent of enzymatic hydrolysis of cellulosic substrates, α-cellulose was hydrolysed using a commercial cellulase preparation at ...

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Effect of Agitation Speed for Enzymatic Hydrolysis of ...

productivity of hydrolysis process because the enzyme is sensitive to mechanical shear stress. Thus, the mixing efficiency should be considered in the optimization process. For this reasons, the aim of this work was to investigate the effect of agitation speed for enzymatic hydrolysis of tapioca

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Effects of Enzyme Concentration, Temperature, pH and Time ...

Hydrolysis of protein was done by enzymatic hydrolysis using alcalase, an alkaline bacterial protease as a biocatalyst. Approximately 6 g of freeze dried PE were dissolved in 100 mL of phosphate buffers (0.1 M, pH7, 8 and 9) and heated at 95°C for 15 min. The hydrolysis was carried out by mixing 50 ml of protein solutions with 50

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Enzymatic hydrolysis of bacterial cellulose in the ...

Nov 08, 2021  2.3 Enzymatic hydrolysis of bacterial cellulose. To evaluate the boosting effect of the CP on the degradation of BC, the enzymatic hydrolysis of the cellulosic substrate was performed using cellulase enzyme with and without the addition of the non-catalytic CP.

Get Price

EFFECT OF PRETREATMENT ON ENZYMATIC HYDROLYSIS OF ...

Bagasse and chopped poplar wood were used as substrates for studying the effects of pretreat-ments on enzymatic hydrolysis of cellulose. Pretreatments by acid ,alkali and steam-explosion and enzymatichydrolysis at 50℃ for 24 h were adopted in the experiments, Pretreated materials were analysed by de-terming total sugar,sugar fraction and ...

Get Price

Hydrothermal pretreatment and enzymatic hydrolysis of ...

In this study effect of mixing on the yields from hydrothermal pretreatment and enzymatic hydrolysis was analyzed by mixing three different lignocellulosic residues - Bermuda grass, Jasmine hedges, and date palm fronds. Results showed that the individual and the mixed lignocellulosics gave same yields when treated under similar conditions of ...

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The effect of shaking regime on the rate and extent of ...

In an attempt to elucidate the effect of mixing on the rate and extent of enzymatic hydrolysis of cellulosic substrates, alpha -cellulose was hydrolysed using a commercial cellulase preparation at varying levels of substrate concentration (2.5,5 and 7.5% (w/v)) and by using three shaking regimes: continuous at low-speed (25 rpm), continuous at high-speed (150 rpm) and an intermittent regime ...

Get Price

Effect of hemicellulose and lignin removal on enzymatic ...

T1 - Effect of hemicellulose and lignin removal on enzymatic hydrolysis of steam pretreated corn stover. AU - Öhgren, Karin. AU - Bura, Renata. AU - Saddler, Jack. AU - Zacchi, Guido. PY - 2007. Y1 - 2007. N2 - Ethanol can be produced from lignocellulosic biomass using steam pretreatment followed by enzymatic hydrolysis and fermentation.

Get Price
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